Punch biopsies taken 12, 24, 48, and 72 hours after skin testing with Leprosin A have been used to prepare ultrathin sections for the identification and enumeration of infiltration cells. The study was performed on small numbers of both healthy persons and leprosy patients with various forms of the disease living in India. Similar cells were found to infiltrate both positive and negative responses to the skin test reagent, although there were quantitative differences. The most striking findings were the absence of the expected basophils and an infiltration of eosinophils which proceeded to degranulate. This was especially noticeable in a healthy leprosy contact and in patients at the tuberculoid end of the leprosy spectrum, whether or not they produced positive skin reactions to Leprosin A. In patients at the lepromatous end of the spectrum, infiltrates were largely neutrophils.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 - http://www.ncbi.nlm.nih.gov/pubmed/3900246?dopt=Abstract DA - 1985 Sep IS - 3 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -Punch biopsies taken 12, 24, 48, and 72 hours after skin testing with Leprosin A have been used to prepare ultrathin sections for the identification and enumeration of infiltration cells. The study was performed on small numbers of both healthy persons and leprosy patients with various forms of the disease living in India. Similar cells were found to infiltrate both positive and negative responses to the skin test reagent, although there were quantitative differences. The most striking findings were the absence of the expected basophils and an infiltration of eosinophils which proceeded to degranulate. This was especially noticeable in a healthy leprosy contact and in patients at the tuberculoid end of the leprosy spectrum, whether or not they produced positive skin reactions to Leprosin A. In patients at the lepromatous end of the spectrum, infiltrates were largely neutrophils.
PY - 1985 SP - 395 EP - 403 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Microscopic findings of delayed reactions elicited by the skin test reagent Leprosin A derived from M. leprae. VL - 53 SN - 0148-916X ER -