TY - JOUR
KW - Autoradiography
KW - Cells, Cultured
KW - DNA, Bacterial
KW - Infection
KW - leprosy
KW - Macrophages
KW - Microscopy, Electron
KW - Monocytes
KW - Mycobacterium leprae
KW - Staining and Labeling
KW - Thymidine
KW - Tritium
AU - Talwar G P
AU - Krishnan A D
AU - Gupta P D
AB - <p>Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-(3)H]thymidine into deoxyribonucleic acid of the bacterial cells. The radioactive precursor is not taken up by the host cells, and evidence has been presented for its incorporation into bacteria. The procedure is sensitive, reproducible, and highly quantitative.</p>
BT - Infection and immunity
C1 - http://www.ncbi.nlm.nih.gov/pubmed/4128631?dopt=Abstract
DA - 1974 Jan
IS - 1
J2 - Infect. Immun.
LA - eng
N2 - <p>Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-(3)H]thymidine into deoxyribonucleic acid of the bacterial cells. The radioactive precursor is not taken up by the host cells, and evidence has been presented for its incorporation into bacteria. The procedure is sensitive, reproducible, and highly quantitative.</p>
PY - 1974
SP - 187
EP - 91
T2 - Infection and immunity
TI - Quantitative evaluation of the progress of intracellular infection in vitro: incorporation of 3H-thymidine into deoxyribonucleic acid by Mycobacterium leprae in cultivated blood monocytes.
UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC414783/pdf/iai00241-0199.pdf
VL - 9
SN - 0019-9567
ER -