TY - JOUR
KW - Blotting, Western
KW - Brazil
KW - Enzyme-Linked Immunosorbent Assay
KW - Evaluation Studies as Topic
KW - Fluorescent Antibody Technique
KW - HIV Antibodies
KW - Hemagglutination Tests
KW - Humans
KW - Sensitivity and Specificity
AU - Ivo-Dos-Santos J
AU - Mello D L
AU - Couto-Fernandez J C
AU - Passos R M
AU - Dias-Carneiro L A
AU - Castilho E A
AU - Galvão-Castro B
AB - <p>Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.</p>
BT - Revista do Instituto de Medicina Tropical de Sao Paulo
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2095632?dopt=Abstract
DA - 1990 Mar-Apr
DO - 10.1590/s0036-46651990000200006
IS - 2
J2 - Rev. Inst. Med. Trop. Sao Paulo
LA - eng
N2 - <p>Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.</p>
PY - 1990
SP - 96
EP - 100
T2 - Revista do Instituto de Medicina Tropical de Sao Paulo
TI - Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brazilian sera.
VL - 32
SN - 0036-4665
ER -