A method is described for the extraction of mycolic acid from lepromatous leprosy tissues and from tuberculous tissues and its identification by thin layer chromatography. Verification of the mycolic acid extracted and separated by thin layer chromatography was accomplished by infrared spectroscopy. Although the presence of mycolic acid in tissues is not, in itself, diagnostic for leprosy, its demonstration in biopsy tissues might be a useful additional qualitative test if used in conjunction with clinical evidence of the disease.
BT - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association C1 - http://www.ncbi.nlm.nih.gov/pubmed/573749?dopt=Abstract DA - 1979 Sep IS - 3 J2 - Int. J. Lepr. Other Mycobact. Dis. LA - eng N2 -A method is described for the extraction of mycolic acid from lepromatous leprosy tissues and from tuberculous tissues and its identification by thin layer chromatography. Verification of the mycolic acid extracted and separated by thin layer chromatography was accomplished by infrared spectroscopy. Although the presence of mycolic acid in tissues is not, in itself, diagnostic for leprosy, its demonstration in biopsy tissues might be a useful additional qualitative test if used in conjunction with clinical evidence of the disease.
PY - 1979 SP - 484 EP - 6 T2 - International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association TI - Detection of mycolic acid in leprous tissue. VL - 47 SN - 0148-916X ER -