TY - JOUR
KW - Amino Acid Sequence
KW - Antibodies, Bacterial
KW - Antibody Specificity
KW - Antigen-Antibody Reactions
KW - Antigens, Bacterial
KW - B-Lymphocytes
KW - Binding Sites, Antibody
KW - Enzyme-Linked Immunosorbent Assay
KW - Epitopes
KW - Ethiopia
KW - Humans
KW - Leprosy, Borderline
KW - Leprosy, lepromatous
KW - Leprosy, Tuberculoid
KW - Longitudinal studies
KW - Molecular Sequence Data
KW - Mycobacterium leprae
KW - Peptide Fragments
KW - Peptide Mapping
AU - Filley E
AU - Thole J E
AU - Rook G A
AU - Nagai S
AU - Waters M
AU - Drijfhout J W
AU - Rinke de Wit T F
AU - Vries R R
AU - Abou-Zeid C
AB - <p>Sixty-three overlapping 15-oligomer peptides covering the 30-kDa protein antigen 85B of Mycobacterium leprae were tested by ELISA to identify epitopes recognized by human antibodies. Serum samples from patients with lepromatous leprosy (LL) reacted mainly with peptides comprising amino acid regions (AA) 206-230, 251-280, and 291-325. Sera of patients with active tuberculosis who responded to the native 30-kDa antigen did not recognize these peptides. The antibody-binding specificity to the defined B cell regions was evaluated in a blind study with 71 serum samples from patients and household contacts living in Ethiopia where leprosy is endemic. The peptide of AA 256-280 was recognized by 88% of LL patients, 15% of patients with tuberculoid leprosy, and none of the contacts. These findings suggest that there are major linear B cell epitopes on the M. leprae 30-kDa protein that are recognized by lepromin-negative LL patients, whereas lepromin-positive patients respond preferentially to conformational epitopes.</p>
BT - The Journal of infectious diseases
C1 - http://www.ncbi.nlm.nih.gov/pubmed/7506279?dopt=Abstract
DA - 1994 Jan
DO - 10.1093/infdis/169.1.162
IS - 1
J2 - J. Infect. Dis.
LA - eng
N2 - <p>Sixty-three overlapping 15-oligomer peptides covering the 30-kDa protein antigen 85B of Mycobacterium leprae were tested by ELISA to identify epitopes recognized by human antibodies. Serum samples from patients with lepromatous leprosy (LL) reacted mainly with peptides comprising amino acid regions (AA) 206-230, 251-280, and 291-325. Sera of patients with active tuberculosis who responded to the native 30-kDa antigen did not recognize these peptides. The antibody-binding specificity to the defined B cell regions was evaluated in a blind study with 71 serum samples from patients and household contacts living in Ethiopia where leprosy is endemic. The peptide of AA 256-280 was recognized by 88% of LL patients, 15% of patients with tuberculoid leprosy, and none of the contacts. These findings suggest that there are major linear B cell epitopes on the M. leprae 30-kDa protein that are recognized by lepromin-negative LL patients, whereas lepromin-positive patients respond preferentially to conformational epitopes.</p>
PY - 1994
SP - 162
EP - 9
T2 - The Journal of infectious diseases
TI - Identification of an antigenic domain on Mycobacterium leprae protein antigen 85B, which is specifically recognized by antibodies from patients with leprosy.
VL - 169
SN - 0022-1899
ER -