TY - JOUR KW - Antigens, Bacterial KW - Cells, Cultured KW - Humans KW - Latex KW - leprosy KW - Microspheres KW - Monocytes KW - Phagocytosis KW - Superoxides AU - Hasan R S AU - Dockrell H M AU - Jamil S AU - Chiang T J AU - Hussain R AB -

To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of MLSON-coated particles was greater than that of control particles in monocytes from both leprosy patients and controls from leprosy-endemic areas; uptake of MLSON-coated particles was higher in monocytes from lepromatous leprosy patients than in cells from tuberculoid leprosy patients and controls. In both patients and controls, uptake of latex particles coated with leprosy antigens triggered very little reduction of nitroblue tetrazolium although the cells were capable of mounting a respiratory burst. Antigen-coated latex particles can therefore be used as a tool to investigate monocyte responses to M. leprae and individual recombinant antigens.

BT - Infection and immunity C1 - http://www.ncbi.nlm.nih.gov/pubmed/8395470?dopt=Abstract DA - 1993 Sep IS - 9 J2 - Infect. Immun. LA - eng N2 -

To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of MLSON-coated particles was greater than that of control particles in monocytes from both leprosy patients and controls from leprosy-endemic areas; uptake of MLSON-coated particles was higher in monocytes from lepromatous leprosy patients than in cells from tuberculoid leprosy patients and controls. In both patients and controls, uptake of latex particles coated with leprosy antigens triggered very little reduction of nitroblue tetrazolium although the cells were capable of mounting a respiratory burst. Antigen-coated latex particles can therefore be used as a tool to investigate monocyte responses to M. leprae and individual recombinant antigens.

PY - 1993 SP - 3724 EP - 9 T2 - Infection and immunity TI - Antigen-coated latex particles as a model system for probing monocyte responses in leprosy. VL - 61 SN - 0019-9567 ER -