TY - JOUR
KW - Antigens, Bacterial
KW - Cells, Cultured
KW - Heat-Shock Proteins
KW - Humans
KW - In Vitro Techniques
KW - Interleukin-6
KW - Lepromin
KW - Leprosy, lepromatous
KW - Leprosy, Tuberculoid
KW - Mycobacterium
AU - Launois P
AU - Vandenbussche P
AU - M'Bayame N N
AU - Drowart A
AU - Van Vooren J P
AU - Sarthou J L
AU - Millan J
AU - Huygen K
AB - <p>IL-6 production was examined in PBMC cultures from healthy leprosy contacts and from leprosy patients stimulated with the purified mycobacterial 18-, 65-, and 70-kDa heat-shock proteins (hsp) and the secreted fibronectin-binding antigen 85 (Ag85). In lepromin-negative contacts, the 70-kDa hsp was the only antigen capable of eliciting significant IL-6 production. In lepromin-positive contacts, Ag85, the 65- and the 70-kDa hsp induced substantial IL-6 titers. IL-6 levels induced with the 70-kDa antigen were about fourfold higher than with the 65-kDa hsp or with Ag85. The 18-kDa antigen did not induce any IL-6 in these healthy contacts. PBMC from tuberculoid leprosy patients produced even more elevated levels of IL-6, and PBMC from lepromatous leprosy patients produced extremely high levels of IL-6. All antigens were capable of inducing IL-6 in leprosy patients. Highest levels were found in cultures stimulated with the 65-kDa hsp, and lowest levels were in cultures stimulated with the 18-kDa hsp.</p>
BT - Cellular immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/8495494?dopt=Abstract
DA - 1993 May
DO - 10.1006/cimm.1993.1112
IS - 2
J2 - Cell. Immunol.
LA - eng
N2 - <p>IL-6 production was examined in PBMC cultures from healthy leprosy contacts and from leprosy patients stimulated with the purified mycobacterial 18-, 65-, and 70-kDa heat-shock proteins (hsp) and the secreted fibronectin-binding antigen 85 (Ag85). In lepromin-negative contacts, the 70-kDa hsp was the only antigen capable of eliciting significant IL-6 production. In lepromin-positive contacts, Ag85, the 65- and the 70-kDa hsp induced substantial IL-6 titers. IL-6 levels induced with the 70-kDa antigen were about fourfold higher than with the 65-kDa hsp or with Ag85. The 18-kDa antigen did not induce any IL-6 in these healthy contacts. PBMC from tuberculoid leprosy patients produced even more elevated levels of IL-6, and PBMC from lepromatous leprosy patients produced extremely high levels of IL-6. All antigens were capable of inducing IL-6 in leprosy patients. Highest levels were found in cultures stimulated with the 65-kDa hsp, and lowest levels were in cultures stimulated with the 18-kDa hsp.</p>
PY - 1993
SP - 283
EP - 90
T2 - Cellular immunology
TI - IL-6 production in response to purified mycobacterial heat-shock proteins and to antigen 85 in leprosy.
VL - 148
SN - 0008-8749
ER -