TY - JOUR
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Antibodies, Bacterial
KW - Antigens, Bacterial
KW - Chaperonin 10
KW - Cross Reactions
KW - Humans
KW - leprosy
KW - Middle Aged
KW - Mycobacterium leprae
KW - Sensitivity and Specificity
KW - Tuberculosis
AU - Rojas R E
AU - Demichelis S O
AU - Gimenez M F
AU - Molinari M L
AU - Segal-Eiras A
AB - <p>The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed: within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.</p>
BT - Medicina
C1 - http://www.ncbi.nlm.nih.gov/pubmed/9674227?dopt=Abstract
DA - 1997
IS - 5
J2 - Medicina (B Aires)
LA - eng
N2 - <p>The response to recombinant 10-kD heat shock protein (HSP) of Mycobacterium leprae (rML10) was evaluated by indirect ELISA in sera from leprosy patients, household contacts, tuberculosis patients and healthy controls in a leprosy-endemic area in the North East of Argentina. Some technical parameters were analyzed: within-assay and between-assay variability, dose-response curves and detectability indexes (specificity and sensitivity) of ELISA applied to measure anti-10 kDa antibodies. High levels of these antibodies have already been reported in positive bacilloscopy patients; herein we have also demonstrated that tuberculosis patients sera cross-react with this M. leprae antigen. This test seems to have a low sensitivity and specificity for leprosy detection; it confirms that antibodies against highly conserved HSP antigens are important in the polyclonal response against mycobacterial epitopes in leprosy as well as in tuberculosis.</p>
PY - 1997
SP - 581
EP - 6
T2 - Medicina
TI - Cross-reactivity of anti-10kD heat shock protein antibodies in leprosy and tuberculosis patients.
VL - 57
SN - 0025-7680
ER -