TY - JOUR
KW - Adult
KW - Aged
KW - Alleles
KW - Cytochrome P-450 CYP2E1
KW - Female
KW - Genetic Predisposition to Disease
KW - genotype
KW - Glutathione Transferase
KW - Humans
KW - leprosy
KW - Male
KW - Middle Aged
KW - Polymorphism, Single Nucleotide
KW - Risk Factors
AU - Pinto P
AU - Salgado CG
AU - Santos N
AU - Alencar D
AU - Santos S
AU - Hutz M
AU - Ribeiro-Dos-Santos A
AB - <p><b>BACKGROUND: </b>The CYP2E1 and GSTM1 genes encode metabolic enzymes that have key functions in drug modification and elimination.</p><p><b>METHODOLOGY/PRINCIPAL FINDINGS: </b>We investigated the possible effects of CYP2E1 and GSTM1 polymorphisms in 71 leprosy patients and in 110 individuals from the general population. The GSTM1*0 null allele and INDEL CYP2E1*1D mutant genotypes were analyzed by conventional PCR, while CYP2E1 SNPs (1053C>T, 1293G>C and 7632T>A) were determined by RT-PCR. In leprosy patients, the GSTM1*0 and CYP2E1*5 alleles and the combined alleles GSTM1*0/CYP2E1*6 and GSTM1*0/CYP2E1*5 were significantly related to a baciloscopic index (BI) (BI<3), while the CYP2E1*6 allele was related to a better clinical evolution in the leprosy spectrum.</p><p><b>CONCLUSIONS/SIGNIFICANCE: </b>Therefore, GSTM1*0, CYP2E1*5 and CYP2E1*6 may be possible protection factors for leprosy patients.</p>
BT - PloS one
C1 - http://www.ncbi.nlm.nih.gov/pubmed/23077626?dopt=Abstract
CN - PINTO 2012
DA - 2012
DO - 10.1371/journal.pone.0047498
IS - 10
J2 - PLoS ONE
LA - eng
N2 - <p><b>BACKGROUND: </b>The CYP2E1 and GSTM1 genes encode metabolic enzymes that have key functions in drug modification and elimination.</p><p><b>METHODOLOGY/PRINCIPAL FINDINGS: </b>We investigated the possible effects of CYP2E1 and GSTM1 polymorphisms in 71 leprosy patients and in 110 individuals from the general population. The GSTM1*0 null allele and INDEL CYP2E1*1D mutant genotypes were analyzed by conventional PCR, while CYP2E1 SNPs (1053C>T, 1293G>C and 7632T>A) were determined by RT-PCR. In leprosy patients, the GSTM1*0 and CYP2E1*5 alleles and the combined alleles GSTM1*0/CYP2E1*6 and GSTM1*0/CYP2E1*5 were significantly related to a baciloscopic index (BI) (BI<3), while the CYP2E1*6 allele was related to a better clinical evolution in the leprosy spectrum.</p><p><b>CONCLUSIONS/SIGNIFICANCE: </b>Therefore, GSTM1*0, CYP2E1*5 and CYP2E1*6 may be possible protection factors for leprosy patients.</p>
PY - 2012
EP - e47498
T2 - PloS one
TI - Polymorphisms in the CYP2E1 and GSTM1 genes as possible protection factors for leprosy patients.
UR - http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0047498
VL - 7
SN - 1932-6203
ER -