Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South-East Asian strains of M. leprae. This article is protected by copyright. All rights reserved.
BT - Clinical microbiology and infection C1 -http://www.ncbi.nlm.nih.gov/pubmed/24612452?dopt=Abstract
DA - 2014 Feb 25 DO - 10.1111/1469-0691.12609 J2 - Clin. Microbiol. Infect. LA - eng N2 -Genotyping and molecular characterization of drug resistance mechanisms in Mycobacterium leprae enables disease transmission and drug resistance trends to be monitored. In the present study, we performed genome-wide analysis of Airaku-3, a multidrug resistant strain with an unknown mechanism of resistance to rifampicin. We identified 12 unique non-synonymous single-nucleotide polymorphisms (SNPs) including two in the transporter-encoding ctpC and ctpI genes. In addition, two SNPs were found that improve the resolution of SNP-based genotyping, particularly for Venezuelan and South-East Asian strains of M. leprae. This article is protected by copyright. All rights reserved.
PY - 2014 T2 - Clinical microbiology and infection TI - Genome-wide re-sequencing of multi-drug resistant Mycobacterium leprae Airaku-3. UR - http://www.clinicalmicrobiologyandinfection.com/article/S1198-743X(14)65395-7/pdf SN - 1469-0691 ER -