TY - JOUR KW - Young Adult KW - Skin Diseases KW - Skin KW - Sensitivity and Specificity KW - Real-Time Polymerase Chain Reaction KW - Paraffin Embedding KW - Mycobacterium leprae KW - Middle Aged KW - Male KW - Leprosy, Paucibacillary KW - Interspersed Repetitive Sequences KW - Humans KW - Female KW - DNA, Bacterial KW - Child KW - Case-Control Studies KW - Biopsy KW - Bacterial Load KW - Aged KW - Adult KW - Adolescent AU - Yan W AU - Xing Y AU - Yuan LC AU - Yang R AU - Tan F AU - Zhang Y AU - Li H AB -

The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.

BT - The American journal of tropical medicine and hygiene C1 -

http://www.ncbi.nlm.nih.gov/pubmed/24493677?dopt=Abstract

DA - 2014 Mar DO - 10.1371/journal.pntd.0002845 IS - 3 J2 - Am. J. Trop. Med. Hyg. LA - eng N2 -

The TaqMan real-time polymerase chain reaction (PCR) assay was evaluated systematically with respect to the standard curve, linear range, and used for detecting Mycobacterium leprae DNA in paraffin-embedded skin biopsy specimens from 60 confirmed leprosy patients and three healthy individuals and 29 other dermatoses and bacterial DNA from 21 different species. The test was further evaluated with 51 paucibacillary (PB) patients. The results showed that the test had good sensitivity (8 fg) and good specificity with no cross-reactivity with 21 other bacterial species and the control specimens, except one with Xanthomatosis. The real-time PCR detection rate for the 51 PB specimens was 74.5% (38 of 51). We conclude that the real-time PCR test is a useful adjunct test for diagnosing early stage or PB leprosy cases.

PY - 2014 SP - 524 EP - 9 T2 - The American journal of tropical medicine and hygiene TI - Application of RLEP real-time PCR for detection of M. leprae DNA in paraffin-embedded skin biopsy specimens for diagnosis of paucibacillary leprosy. UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3945699/pdf/tropmed-90-524.pdf VL - 90 SN - 1476-1645 ER -