To study the patterns of nerve involvement in tuberculoid leprosy using S-100 immunostain and to compare its sensitivity with that of hematoxylin and eosin stain. The paraffin sections of twenty five cases of leprosy including five of tuberculoid (TT) and 20 of borderline tuberculoid (BT) type were subjected to both H& E and S-100 immunostaining. The patterns of nerve involvement on S-100 immunostaining were graded as infiltrated (A), fragmented (B), absent (C) and intact (D). On H&E staining only 2/5 (40%) TT cases and 9/20 (45%) of BT cases revealed neural inflammation. On S-100 staining of TT cases, 3/5 (60%) showed pattern B of nerve involvement followed by pattern A and C in 1/5 (20%) and 1/5 (20%) cases respectively. Out of the 20 cases of BT, S-100 demonstrated B pattern of nerve involvement in 14 (70%), A in 5 (25%) and C in 1 (5%). S-100 Stain is an efficient ancillary aid in histopathological diagnosis of tuberculoid pole of leprosy and is superior to H&E stain in identifying the neural inflammation in granulomas. The fragmented pattern of nerve involvement was the most common pattern seen in both TT (60%) and BT (70%) cases.
BT - JK science : journal of medical education and research IS - 2 J2 - JK Science LA - eng N2 -To study the patterns of nerve involvement in tuberculoid leprosy using S-100 immunostain and to compare its sensitivity with that of hematoxylin and eosin stain. The paraffin sections of twenty five cases of leprosy including five of tuberculoid (TT) and 20 of borderline tuberculoid (BT) type were subjected to both H& E and S-100 immunostaining. The patterns of nerve involvement on S-100 immunostaining were graded as infiltrated (A), fragmented (B), absent (C) and intact (D). On H&E staining only 2/5 (40%) TT cases and 9/20 (45%) of BT cases revealed neural inflammation. On S-100 staining of TT cases, 3/5 (60%) showed pattern B of nerve involvement followed by pattern A and C in 1/5 (20%) and 1/5 (20%) cases respectively. Out of the 20 cases of BT, S-100 demonstrated B pattern of nerve involvement in 14 (70%), A in 5 (25%) and C in 1 (5%). S-100 Stain is an efficient ancillary aid in histopathological diagnosis of tuberculoid pole of leprosy and is superior to H&E stain in identifying the neural inflammation in granulomas. The fragmented pattern of nerve involvement was the most common pattern seen in both TT (60%) and BT (70%) cases.
PY - 2017 SP - 103 EP - 106 T2 - JK science : journal of medical education and research TI - S-100 immmunostain as an ancillary aid in the diagnosis of tuberculoid leprosy. UR - http://www.jkscience.org/archives/volume192/9-Original%20Article.pdf VL - 19 ER -