Type 2 reaction (T2R) or (ENL), a sudden episode of acute inflammation predominantly affecting lepromatous leprosy patients (LL), characterized by a reduced cellular immune response. This possibly indicates a close relationship between the onset of T2R and the altered frequency, and functional activity of T lymphocytes, particularly of memory subsets. This study performed ex vivo and in vitro characterizations of T cell blood subpopulations from LL patients with or without T2R. In addition, the evaluation of activity of these subpopulations was performed by analyzing the frequency of these cells producing IFN-γ, TNF, and IL-10 by flow cytometry. Furthermore, the expression of transcription factors, for the differentiation of T cells, were analyzed by quantitative real-time polymerase chain reaction. Our results showed an increased frequency of CD8/TNF effector memory T cells (T) among T2Rs. Moreover, there was evidence of a reduced frequency of CD4 and CD8 IFN-γ-producing cells in T2R, and a reduced expression of and . Finally, a significant and positive correlation between bacillary index (BI) of T2R patients and CD4/TNF and CD4/IFN-γ T cells was observed. Thus, negative correlation between BI and the frequency of CD4/IL-10 T cells was noted. These results suggest that CD8/TNF T are primarily responsible for the transient alteration in the immune response to in ENL patients. Thus, our study will provide a better understanding of pathogenic mechanisms and will lead to new promising therapeutic choices.
BT - The American journal of tropical medicine and hygiene C1 -http://www.ncbi.nlm.nih.gov/pubmed/30652669?dopt=Abstract
DO - 10.4269/ajtmh.18-0517 J2 - Am. J. Trop. Med. Hyg. LA - eng N2 -Type 2 reaction (T2R) or (ENL), a sudden episode of acute inflammation predominantly affecting lepromatous leprosy patients (LL), characterized by a reduced cellular immune response. This possibly indicates a close relationship between the onset of T2R and the altered frequency, and functional activity of T lymphocytes, particularly of memory subsets. This study performed ex vivo and in vitro characterizations of T cell blood subpopulations from LL patients with or without T2R. In addition, the evaluation of activity of these subpopulations was performed by analyzing the frequency of these cells producing IFN-γ, TNF, and IL-10 by flow cytometry. Furthermore, the expression of transcription factors, for the differentiation of T cells, were analyzed by quantitative real-time polymerase chain reaction. Our results showed an increased frequency of CD8/TNF effector memory T cells (T) among T2Rs. Moreover, there was evidence of a reduced frequency of CD4 and CD8 IFN-γ-producing cells in T2R, and a reduced expression of and . Finally, a significant and positive correlation between bacillary index (BI) of T2R patients and CD4/TNF and CD4/IFN-γ T cells was observed. Thus, negative correlation between BI and the frequency of CD4/IL-10 T cells was noted. These results suggest that CD8/TNF T are primarily responsible for the transient alteration in the immune response to in ENL patients. Thus, our study will provide a better understanding of pathogenic mechanisms and will lead to new promising therapeutic choices.
PY - 2019 T2 - The American journal of tropical medicine and hygiene TI - Involvement of TNF-Producing CD8 Effector Memory T Cells with Immunopathogenesis of Erythema Nodosum Leprosum in Leprosy Patients. SN - 1476-1645 ER -