TY - JOUR
KW - Antigens, Bacterial
KW - Antigens, Surface
KW - CD4-Positive T-Lymphocytes
KW - CD40 Ligand
KW - CD8-Positive T-Lymphocytes
KW - Dendritic Cells
KW - Humans
KW - Interferon-gamma
KW - Interleukin-12
KW - Lymphocyte Activation
KW - Membrane Glycoproteins
KW - Mycobacterium leprae
KW - Perforin
KW - Pore Forming Cytotoxic Proteins
AU - Maeda Y
AU - Gidoh M
AU - Ishii N
AU - Mukai C
AU - Makino M
AB - <p>The antigenicity of Mycobacterium leprae (M. leprae)-derived cell membrane fraction was examined using human dendritic cells (DCs). Immature DCs internalized and processed the cell membrane components, and expressed M. leprae-derived antigens (Ags) on their surface. The expression of MHC class II, CD86, and CD83 Ags on DCs and CD40 ligand (L)-associated IL-12 p70 production from DCs were up-regulated by the membrane Ags. Moreover these stimulated DCs induced significantly higher level of interferon-gamma (IFN-gamma) production by autologous CD4(+) and CD8(+) T cells than those pulsed with equivalent doses of live M. leprae or its cytosol fraction. Both subsets of T cells from tuberculoid leprosy patients also produced several fold more IFN-gamma than those from normal individuals. Furthermore, the intracellular perforin production in CD8(+) T cells was up-regulated in an Ag-dose dependent manner. These results suggest that M. leprae membrane Ags might be useful as the vaccinating agents against leprosy.</p>
BT - Cellular immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/12798309?dopt=Abstract
DA - 2003 Mar
DO - 10.1016/s0008-8749(03)00078-9
IS - 1
J2 - Cell. Immunol.
LA - eng
N2 - <p>The antigenicity of Mycobacterium leprae (M. leprae)-derived cell membrane fraction was examined using human dendritic cells (DCs). Immature DCs internalized and processed the cell membrane components, and expressed M. leprae-derived antigens (Ags) on their surface. The expression of MHC class II, CD86, and CD83 Ags on DCs and CD40 ligand (L)-associated IL-12 p70 production from DCs were up-regulated by the membrane Ags. Moreover these stimulated DCs induced significantly higher level of interferon-gamma (IFN-gamma) production by autologous CD4(+) and CD8(+) T cells than those pulsed with equivalent doses of live M. leprae or its cytosol fraction. Both subsets of T cells from tuberculoid leprosy patients also produced several fold more IFN-gamma than those from normal individuals. Furthermore, the intracellular perforin production in CD8(+) T cells was up-regulated in an Ag-dose dependent manner. These results suggest that M. leprae membrane Ags might be useful as the vaccinating agents against leprosy.</p>
PY - 2003
SP - 69
EP - 77
T2 - Cellular immunology
TI - Assessment of cell mediated immunogenicity of Mycobacterium leprae-derived antigens.
VL - 222
SN - 0008-8749
ER -