TY - JOUR
KW - Biopsy
KW - DNA, Bacterial
KW - Genes, Bacterial
KW - Humans
KW - leprosy
KW - Mycobacterium leprae
KW - polymerase chain reaction
KW - Polymorphism, Genetic
KW - Sequence Analysis, DNA
KW - Skin
KW - Tandem Repeat Sequences
KW - Thailand
AU - Srisungngam S
AU - Rudeeaneksin J
AU - Wattanpokayakit S
AU - Pasadorn S
AU - Tragoolpua R
AU - Suwanrit S
AU - Sawanpanyalert P
AU - Phetsuksiri B
AB - <p>Mycobacterium leprae isolates from Thai leprosy patients were typed for strain differentiation and analysis of leprosy transmission using the six base tandem repeat, GACATC, in rpoT gene and TTC repeat as genetic markers. M. leprae DNA was isolated from skin biopsies of new untreated leprosy patients living in remote areas or in suburban regions of Thailand where leprosy is in low prevalence. In M. leprae strains of 100 patients, TTC alleles exhibited variations in length with 10 to 30, 33 and 35 repeats, the most common alleles being 15, 16, 17 and 19 repeats. All isolates contained three copies of the six base repeat in rpoT gene. Application of TTC repeats in tracking leprosy transmission in two families with multi-cases identified a single (but different) strain of M. leprae in each family.</p>
BT - The Southeast Asian journal of tropical medicine and public health
C1 - http://www.ncbi.nlm.nih.gov/pubmed/17883012?dopt=Abstract
DA - 2007 Jul
IS - 4
J2 - Southeast Asian J. Trop. Med. Public Health
LA - eng
N2 - <p>Mycobacterium leprae isolates from Thai leprosy patients were typed for strain differentiation and analysis of leprosy transmission using the six base tandem repeat, GACATC, in rpoT gene and TTC repeat as genetic markers. M. leprae DNA was isolated from skin biopsies of new untreated leprosy patients living in remote areas or in suburban regions of Thailand where leprosy is in low prevalence. In M. leprae strains of 100 patients, TTC alleles exhibited variations in length with 10 to 30, 33 and 35 repeats, the most common alleles being 15, 16, 17 and 19 repeats. All isolates contained three copies of the six base repeat in rpoT gene. Application of TTC repeats in tracking leprosy transmission in two families with multi-cases identified a single (but different) strain of M. leprae in each family.</p>
PY - 2007
SP - 714
EP - 20
T2 - The Southeast Asian journal of tropical medicine and public health
TI - Typing of Thai clinical isolates of Mycobacterium leprae and analysis of leprosy transmission by polymorphism of tandem repeats.
UR - http://www.tm.mahidol.ac.th/seameo/2007_38_4/18-3996.pdf
VL - 38
SN - 0125-1562
ER -