TY - JOUR
KW - Animals
KW - Disease Models, Animal
KW - Foot
KW - Gene Expression Profiling
KW - leprosy
KW - Mycobacterium leprae
KW - Oligonucleotide Array Sequence Analysis
KW - Rats
KW - Rats, Nude
AU - Akama T
AU - Tanigawa K
AU - Kawashima A
AU - Wu H
AU - Ishii N
AU - Suzuki K
AB - <p>Mycobacterium leprae, the causative agent of leprosy, does not grow under in vitro condition, making molecular analysis of this bacterium difficult. For this reason, bacteriological information regarding M. leprae gene function is limited compared with other mycobacterium species. In this study, we performed DNA microarray analysis to clarify the RNA expression profile of the Thai53 strain of M. leprae grown in footpads of hypertensive nude rats (SHR/NCrj-rnu). Of 1605 M. leprae genes, 315 showed signal intensity twofold higher than the median. These genes include Acyl-CoA metabolic enzymes and drug metabolic enzymes, which might be related to the virulence of M. leprae. In addition, consecutive RNA expression profile and in silico analyses enabled identification of possible operons within the M. leprae genome. The present results will shed light on M. leprae gene function and further our understanding of the pathogenesis of leprosy.</p>
BT - Microbial pathogenesis
C1 - http://www.ncbi.nlm.nih.gov/pubmed/20553838?dopt=Abstract
DA - 2010 Oct
DO - 10.1016/j.micpath.2010.05.010
IS - 4
J2 - Microb. Pathog.
LA - eng
N2 - <p>Mycobacterium leprae, the causative agent of leprosy, does not grow under in vitro condition, making molecular analysis of this bacterium difficult. For this reason, bacteriological information regarding M. leprae gene function is limited compared with other mycobacterium species. In this study, we performed DNA microarray analysis to clarify the RNA expression profile of the Thai53 strain of M. leprae grown in footpads of hypertensive nude rats (SHR/NCrj-rnu). Of 1605 M. leprae genes, 315 showed signal intensity twofold higher than the median. These genes include Acyl-CoA metabolic enzymes and drug metabolic enzymes, which might be related to the virulence of M. leprae. In addition, consecutive RNA expression profile and in silico analyses enabled identification of possible operons within the M. leprae genome. The present results will shed light on M. leprae gene function and further our understanding of the pathogenesis of leprosy.</p>
PY - 2010
SP - 181
EP - 5
T2 - Microbial pathogenesis
TI - Analysis of Mycobacterium leprae gene expression using DNA microarray.
VL - 49
SN - 1096-1208
ER -