TY - JOUR
KW - LF tests
KW - Mycobacterium leprae
KW - glycoconjugates
KW - leprosy
KW - serodiagnosis
AU - Korolyova-Ushakova A
AU - Baranova E
AU - Ignatov S
AU - Fedyukina G
AU - Solov'ev P
AU - Kolombet L
AU - Biketov S
AB - <p>The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest). The data obtained with sera of leprosy patients, patients who have been in contact with leprosy, and healthy donors indicate that the most promising antigenic component is a BSA conjugate with two synthetic epitopes - a disaccharide derivative of PGL-1 and a branched hexasaccharide fragment of LAM. The leprosy LF serotest with such glycoconjugate demonstrated the greatest diagnostic sensitivity for main forms of leprosy - paucibacillary (PB) and multibacillary (MB).</p>
BT - Klinicheskaia laboratornaia diagnostika
C1 - https://www.ncbi.nlm.nih.gov/pubmed/32298544
DA - 01/2020
DO - 10.18821/0869-2084-2020-65-5-289-293
IS - 5
J2 - Klin. Lab. Diagn.
LA - rus
N2 - <p>The glycoconjugates with BSA (bovine serum albumin) were synthesized using a next saccharide: disaccharide derivative M.leprae PGL-1 (phenolic glycolipid-1); a complex of the disaccharide fragment and the branched hexasaccharide fragment LAM (lipoarabinomannan); diarabinofuranose fragment LAM. These glycoconjugates were used as antigenic components for leprosy rapid serotest construction in immunochromatographic format (leprosy LF serotest). The data obtained with sera of leprosy patients, patients who have been in contact with leprosy, and healthy donors indicate that the most promising antigenic component is a BSA conjugate with two synthetic epitopes - a disaccharide derivative of PGL-1 and a branched hexasaccharide fragment of LAM. The leprosy LF serotest with such glycoconjugate demonstrated the greatest diagnostic sensitivity for main forms of leprosy - paucibacillary (PB) and multibacillary (MB).</p>
PY - 2020
SP - 289
EP - 293
T2 - Klinicheskaia laboratornaia diagnostika
TI - [The use of synthetic glycoconjugates as components of the immunochromatographic test for rapid serological diagnosis of leprosy.]
VL - 65
SN - 0869-2084
ER -