TY - JOUR
KW - Carbohydrates
KW - diagnosis
KW - glycoconjugates
KW - lateral flow assay
KW - leprosy
AU - van Dijk HJ
AU - van Hooij A
AU - Groot ML
AU - Geboers J
AU - Moretti R
AU - Verhard-Seymonsbergen E
AU - de Jong D
AU - van der Marel G
AU - Corstjens P
AU - Codée J
AU - Geluk A
AB - <p>Point-of-care (POC) diagnostic tests for rapid detection of individuals infected with Mycobacterium leprae ( M. leprae) , the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinical outcome and reduction of transmission of this infectious disease. Levels of antibodies directed against the M. leprae -specific phenolic glycolipid I (PGL-I) closely correlate with an individual's bacterial load and higher risk of developing leprosy. We here describe the assembly of a set of PGL glycans carrying the characteristic phenol aglycon and featuring different methylation patterns. The PGL trisaccharides were applied to construct neoglycoproteins that were used to detect anti-PGL IgM antibodies in leprosy patients. ELISAs and quantitative lateral flow assays based on up-converting nanoparticles (UCP-LFAs) showed that the generated PGL-I and PGL-II trisaccharide neoglycoconjugates can be applied for the detection of anti M. leprae IgM antibodies in POC tests.</p>
BT - Chembiochem : a European journal of chemical biology
C1 - https://www.ncbi.nlm.nih.gov/pubmed/33332701
DA - 12/2020
DO - 10.1002/cbic.202000810
J2 - Chembiochem
LA - eng
N2 - <p>Point-of-care (POC) diagnostic tests for rapid detection of individuals infected with Mycobacterium leprae ( M. leprae) , the causative pathogen of leprosy, represent efficient tools to guide therapeutic and prophylactic treatment strategies in leprosy control programs, thus positively contributing to clinical outcome and reduction of transmission of this infectious disease. Levels of antibodies directed against the M. leprae -specific phenolic glycolipid I (PGL-I) closely correlate with an individual's bacterial load and higher risk of developing leprosy. We here describe the assembly of a set of PGL glycans carrying the characteristic phenol aglycon and featuring different methylation patterns. The PGL trisaccharides were applied to construct neoglycoproteins that were used to detect anti-PGL IgM antibodies in leprosy patients. ELISAs and quantitative lateral flow assays based on up-converting nanoparticles (UCP-LFAs) showed that the generated PGL-I and PGL-II trisaccharide neoglycoconjugates can be applied for the detection of anti M. leprae IgM antibodies in POC tests.</p>
PY - 2020
T2 - Chembiochem : a European journal of chemical biology
TI - Synthetic Phenolic Glycolipids for application in diagnostic tests  for leprosy.
SN - 1439-7633
ER -