Background: The causative agents of leprosy are the well-known and the newly discovered . This agent was found in 2008, and it was found to be the cause of diffuse lepromatous leprosy in two Mexican patients.
Objective: The objective of this work was to determine if and were present in formalin-fixed and paraffin-embedded skin samples from cases from different regions in Mexico.
Methods: A total of 41 skin samples were obtained from 11 states of Mexico. All patients' samples were diagnosed by clinical and histopathological analyses. Total DNA was isolated using a Qiagen-DNeasy blood and tissue kit and molecular identification was achieved by two semi-nested polymerase chain reactions.
Results: The 41 patient included 33 samples from men and 8 samples from women; 29 samples were polymerase chain reaction (PCR)-positive to and 12 samples were PCR-negative. From those 29 samples, 13 were PCR-positive to , 8 to and 8 were positive to both species. The histopathological diagnosis included; Nodular lepromatous leprosy (NLL); Diffuse lepromatous leprosy (DLL); and Borderline leprosy (BL). The 29 PCR-positive samples were classified as follow: 14 NLL, 4 DLL, and 11 BL. In the 12 samples negative to , 7 showed the NLL, 2 DLL and 3 BL.
Conclusion: These findings add evidence to the and distribution, clinical forms and participation of dual infections in Mexico.
BT - Annals of dermatology C1 - https://www.ncbi.nlm.nih.gov/pubmed/33911479 DA - 10/2018 DO - 10.5021/ad.2018.30.5.562 IS - 5 J2 - Ann Dermatol LA - eng N2 -Background: The causative agents of leprosy are the well-known and the newly discovered . This agent was found in 2008, and it was found to be the cause of diffuse lepromatous leprosy in two Mexican patients.
Objective: The objective of this work was to determine if and were present in formalin-fixed and paraffin-embedded skin samples from cases from different regions in Mexico.
Methods: A total of 41 skin samples were obtained from 11 states of Mexico. All patients' samples were diagnosed by clinical and histopathological analyses. Total DNA was isolated using a Qiagen-DNeasy blood and tissue kit and molecular identification was achieved by two semi-nested polymerase chain reactions.
Results: The 41 patient included 33 samples from men and 8 samples from women; 29 samples were polymerase chain reaction (PCR)-positive to and 12 samples were PCR-negative. From those 29 samples, 13 were PCR-positive to , 8 to and 8 were positive to both species. The histopathological diagnosis included; Nodular lepromatous leprosy (NLL); Diffuse lepromatous leprosy (DLL); and Borderline leprosy (BL). The 29 PCR-positive samples were classified as follow: 14 NLL, 4 DLL, and 11 BL. In the 12 samples negative to , 7 showed the NLL, 2 DLL and 3 BL.
Conclusion: These findings add evidence to the and distribution, clinical forms and participation of dual infections in Mexico.
PY - 2018 SP - 562 EP - 565 T2 - Annals of dermatology TI - Identification of and in Formalin-Fixed and Paraffin-Embedded Skin Samples from Mexico. UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7992488/pdf/ad-30-562.pdf VL - 30 SN - 2005-3894 ER -