TY - JOUR
KW - Administration, Cutaneous
KW - Adolescent
KW - Adult
KW - Antigens, Bacterial
KW - BCG Vaccine
KW - Cell Count
KW - Dose-Response Relationship, Immunologic
KW - Humans
KW - In Vitro Techniques
KW - Interleukin-2
KW - Leprosy, lepromatous
KW - Lymphocyte Activation
KW - Middle Aged
KW - Phytohemagglutinins
KW - Recombinant Proteins
KW - T-Lymphocytes
AU - Converse P
AU - Ottenhoff T H
AU - Work Teklemariam S
AU - Hancock G E
AU - Dietz M
AU - Becx-Bleumink M
AU - Wondimu A
AU - Kiessling R
AU - Cohn Z A
AU - Kaplan G
AB - <p>Thirty-one patients with lepromatous leprosy received recombinant interleukin 2 (IL-2) intradermally in doses ranging from 10 to 30 micrograms. Before injection and at time intervals of 2-21 days thereafter, samples of peripheral blood mononuclear cells (PBMC) were obtained. Single or multiple injections (1-3) of IL-2 did not modify the total number of circulating lymphocytes or the number of T cells and the CD4/CD8 T-cell ratio. However, IL-2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4-8 days after intradermal IL-2. Stimulation indices of three- to sevenfold above pre-IL-2 levels were observed with the polyclonal activator phytohaemagglutinin (PHA) and enhanced thymidine incorporation occurred in the presence of antigens to which the patients were already sensitized, such as purified protein derivative and BCG. IL-2 had no effect on the unresponsive state of lepromatous leprosy patient T cells to the antigens of Mycobacterium leprae.</p>
BT - Scandinavian journal of immunology
C1 - http://www.ncbi.nlm.nih.gov/pubmed/2389115?dopt=Abstract
DA - 1990 Aug
DO - 10.1111/j.1365-3083.1990.tb02897.x
IS - 2
J2 - Scand. J. Immunol.
LA - eng
N2 - <p>Thirty-one patients with lepromatous leprosy received recombinant interleukin 2 (IL-2) intradermally in doses ranging from 10 to 30 micrograms. Before injection and at time intervals of 2-21 days thereafter, samples of peripheral blood mononuclear cells (PBMC) were obtained. Single or multiple injections (1-3) of IL-2 did not modify the total number of circulating lymphocytes or the number of T cells and the CD4/CD8 T-cell ratio. However, IL-2 had a pronounced influence on the [3H]thymidine incorporation in response to various stimuli 4-8 days after intradermal IL-2. Stimulation indices of three- to sevenfold above pre-IL-2 levels were observed with the polyclonal activator phytohaemagglutinin (PHA) and enhanced thymidine incorporation occurred in the presence of antigens to which the patients were already sensitized, such as purified protein derivative and BCG. IL-2 had no effect on the unresponsive state of lepromatous leprosy patient T cells to the antigens of Mycobacterium leprae.</p>
PY - 1990
SP - 83
EP - 91
T2 - Scandinavian journal of immunology
TI - Intradermal recombinant interleukin 2 enhances peripheral blood T-cell responses to mitogen and antigens in patients with lepromatous leprosy.
VL - 32
SN - 0300-9475
ER -