Tuberculosis (TB) is a chronic infectious disease caused by infection, which has seriously endangered human health for many years. With the emergence of multidrug-resistant and extensively drug-resistant , the prevention and treatment of TB has become a pressing need. Early diagnosis, drug resistance monitoring, and control of disease transmission are critical aspects in the prevention and treatment of TB. However, the currently available diagnostic technologies and drug sensitivity tests are time consuming, and thus, it is difficult to achieve the goal of early diagnosis and detection drug sensitivity, which results in limited control of disease transmission. The development of molecular testing technology has gradually achieved the vision of rapid and accurate diagnosis of TB. Droplet digital PCR (ddPCR) is an excellent nucleic acid quantification method with high sensitivity and no need for a calibration curve. Herein, we review the application of ddPCR in TB diagnosis and drug resistance detection and transmission monitoring.
BT - Infection and drug resistance C1 -https://www.ncbi.nlm.nih.gov/pubmed/35313727
DA - 01/2022 DO - 10.2147/IDR.S349607 J2 - Infect Drug Resist LA - eng N2 -Tuberculosis (TB) is a chronic infectious disease caused by infection, which has seriously endangered human health for many years. With the emergence of multidrug-resistant and extensively drug-resistant , the prevention and treatment of TB has become a pressing need. Early diagnosis, drug resistance monitoring, and control of disease transmission are critical aspects in the prevention and treatment of TB. However, the currently available diagnostic technologies and drug sensitivity tests are time consuming, and thus, it is difficult to achieve the goal of early diagnosis and detection drug sensitivity, which results in limited control of disease transmission. The development of molecular testing technology has gradually achieved the vision of rapid and accurate diagnosis of TB. Droplet digital PCR (ddPCR) is an excellent nucleic acid quantification method with high sensitivity and no need for a calibration curve. Herein, we review the application of ddPCR in TB diagnosis and drug resistance detection and transmission monitoring.
PY - 2022 SP - 1067 EP - 1076 T2 - Infection and drug resistance TI - Application of Droplet Digital PCR to Detection of Mycobacterium tuberculosis and Mycobacterium leprae Infections: A Narrative Review. UR - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8934166/pdf/idr-15-1067.pdf VL - 15 SN - 1178-6973 ER -