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Soluble mediators of immune significance in sera of leprosy patients
Abstract
Background:
The disease leprosy is categorized by a wide spectrum of clinical forms imposed by the patient’s immune responses to the organism, Mycobacterium leprae. Soluble receptors and soluble mediators are important for regulation of immune responses.
Objectives:
As leprosy reactions are known immune inflammatory reactions during the disease process, the aim of the study was to screen nitrite, IL-6 cytokine and sTLR2 in sera samples to identify circulating biomarkers associated with leprosy and T1R and T2R among patients recruited from the Northern part of India.
Methods:
Leprosy patients recruited in our Institute were included in this study. We analysed the level of nitrite, soluble TLR2 and IL-6 in sera of leprosy patients, healthy controls and healthy household contacts by colorimetric assay and ELISA respectively.
Results:
The level of serum nitrite was observed to be higher in tuberculoid leprosy patients compared to lepromatous leprosy patients (p = 0.006). The level was also higher in healthy contacts of leprosy patients compared to lepromatous leprosy patients (p = 0.008). The sTLR2 level was significantly higher in healthy controls than leprosy patients (p = 0.002) and their healthy contacts (p = 0.001). The level of IL-6 in serum was significantly higher in leprosy patients than in healthy controls (p = 0.001).
Conclusion:
Nitrite, IL-6 cytokine and sTLR2 are shown to be potential circulating biomarkers associated with leprosy among patients recruited in Northern India.
The disease leprosy is categorized by a wide spectrum of clinical forms imposed by the patient’s immune responses to the organism, Mycobacterium leprae. Soluble receptors and soluble mediators are important for regulation of immune responses.
Objectives:
As leprosy reactions are known immune inflammatory reactions during the disease process, the aim of the study was to screen nitrite, IL-6 cytokine and sTLR2 in sera samples to identify circulating biomarkers associated with leprosy and T1R and T2R among patients recruited from the Northern part of India.
Methods:
Leprosy patients recruited in our Institute were included in this study. We analysed the level of nitrite, soluble TLR2 and IL-6 in sera of leprosy patients, healthy controls and healthy household contacts by colorimetric assay and ELISA respectively.
Results:
The level of serum nitrite was observed to be higher in tuberculoid leprosy patients compared to lepromatous leprosy patients (p = 0.006). The level was also higher in healthy contacts of leprosy patients compared to lepromatous leprosy patients (p = 0.008). The sTLR2 level was significantly higher in healthy controls than leprosy patients (p = 0.002) and their healthy contacts (p = 0.001). The level of IL-6 in serum was significantly higher in leprosy patients than in healthy controls (p = 0.001).
Conclusion:
Nitrite, IL-6 cytokine and sTLR2 are shown to be potential circulating biomarkers associated with leprosy among patients recruited in Northern India.
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Type
Journal Article